Determination of exogenous water in grape juice through the isotopic analysis of 18O/16O

Abstract The consumption of grape juice has been growing significantly, so its quality is becoming an issue great importance, both for the consumer and industry. However, identifying adulteration in a challenge requires reliable analytical process. isotope ratio (18O/16O) important tool to determine addition exogenous water beverages, however, there no official method Brazil. This study aimed develop validate detecting through isotopic analysis 18O/16O. development validation were performed using Isotope Ratio Mass Spectrometry (IRMS). effect temperature evaporation ?18O experimental juices was evaluated, reference values found based on ? 18O musts. influence industrial production process verified, commercial evaluated relation regarding water. parameters did not results juice, as they presented differences lower than uncertainty. heat exchanger system proposed method. can come from musts, without affecting interpretation final results. Of thirty real analyzed, nine had water, three proved be reconstituted eighteen considered have validated this limit detection (LOD) 0.24‰, quantification (LOQ) 0.97‰ measurement uncertainty 0.71‰, proving effective 18O/16O by IRMS.